ChIP-MS BAC cells expressing GFP-PICH
CHIP-MS was performed with HeLa cells carrying a bacterial artifical chromosome for the expression of GFP-tagged PICH under it's endogenous promotor. Cells were treated with drugs to induce different types of ultra-fine bridges (UFBs). Treatments included: a) 24h exposure to 0.1 uM Aphidicolin, b) 24 hours exposure to 120 ng/ml mitomycin C, c) 6.5 hours incubation in 0.05 ug/ml nocodazol followed by a release into 0.1 uM ICRF-193 topoisomerase inhibitor.
SEEK ID: https://seek-for2800.de/assays/28
Experimental assay
Projects: SP-6: Proteomics of mitotic inter sister chromatid junctions
Investigation: Identification of factors involved in UFB resolution
Study: hidden item
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Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Created: 8th Feb 2022 at 12:21
Last updated: 8th Feb 2022 at 12:57
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Our Research Unit addresses an important question on the origin of chromosome instability (CIN), which causes structural as well as numerical chromosome aberrations. Importantly, CIN and increased levels of chromosome aberrations are closely associated with many human diseases including cancer, neurodegenerative diseases and age-related syndromes and can act as key drivers for disease development and progression. An important condition that causes structural chromosome instability (S-CIN) leading ...
Projects: SP-3: A statistical modeling approach to identify common triggers for replication stress and mitotic chromosome missegregation, SP-1: Deciphering signaling pathways that promote accurate chromosome segregation after replication stress, SP-2: Molecular mechanisms of replication stress-induced mitotic chromosome missegregation, SP-4: Centrosome integrity as a determinant of replication stress and mitotic dysfunction, SP-5: Impaired chromosome integrity caused by mutations in members of the BTR complex, SP-6: Proteomics of mitotic inter sister chromatid junctions, SP-8: The causes of replication stress in response to whole chromosomal aneuploidy, SP-Z: NGS-based approaches for systematic analysis of genomic and chromosome instability Bernd Wollnik, Göttingen, SP9: Mutual impact of replication origin firing regulation and mitotic chromosome segregation
Web page: https://for2800.de/
Replication stress is a key driver of genomic instability giving rise to human disease, for instance cancer or developmental disorders. It is caused by a variety of defects such as lesions blocking replication (e.g. DNA interstrand crosslinks) or processes that lead to the exhaustion of DNA precursors. This generates under-replicated DNA and replication intermediates that can be rescued by repair processes requiring homologous recombination between sister chromatids. Often, cells exposed to ...
Programme: FOR2800
Public web page: https://for2800.de/cases/sub-project-6-proteomics-of-mitotic-inter-sister-chromatid-junctions/
Replication stress is one of the major causes for chromosomal instability which is a hallmark of cancer. Incomplete repair can lead to severe mitotic defects and ultra-fine anaphase bridges (UFBs) are a typical consequence. So far, five different classes of UFBs have been discribed: fragile sites-UFBs, centromeric-UFBs, telomeric-UFBs, homologous recombination-UFBs and rDNA-UFBs, which can be distinguished by their location and the way how they arise. Although previous studies have already proposed ...
Creator: Angela Wieland
Submitter: Angela Wieland
