Markus Räschle

Replication stress is a key driver of genomic instability giving rise to human disease, for instance cancer or developmental disorders. It is caused by a variety of defects such as lesions blocking replication (e.g. DNA interstrand crosslinks) or processes that lead to the exhaustion of DNA precursors. This generates under-replicated DNA and replication intermediates that can be rescued by repair processes requiring homologous recombination between sister chromatids. Often, cells exposed to ...

CHIP-MS was performed with HeLa cells carrying a bacterial artifical chromosome for the expression of GFP-tagged PICH under it's endogenous promotor. Cells were treated with drugs to induce different types of ultra-fine bridges (UFBs). Treatments included: a) 24h exposure to 0.1 uM Aphidicolin, b) 24 hours exposure to 120 ng/ml mitomycin C, c) 6.5 hours incubation in 0.05 ug/ml nocodazol followed by a release into 0.1 uM ICRF-193 topoisomerase inhibitor.